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Singlet Oxygen in Plants—Its Significance and Possible Detection with Double (Fluorescent and Spin) Indicator Reagents

Identifieur interne : 001722 ( Main/Exploration ); précédent : 001721; suivant : 001723

Singlet Oxygen in Plants—Its Significance and Possible Detection with Double (Fluorescent and Spin) Indicator Reagents

Auteurs : Éva Hideg [Hongrie] ; Tamás Kálai ; Péter B. K S [Hongrie] ; Kozi Asada ; Kálmán Hideg

Source :

Photochemistry and Photobiology [ 0031-8655 ] ; 2006-09.

RBID : ISTEX:AB8F0F1D4BB99A5DD2A4F3E1146452FB1D8992EA

English descriptors

Abstract

Direct detection of reactive oxygen species (ROS), especially singlet oxygen, in plants under stress conditions is of special importance, not only to identify primary events of oxidative damage, but also in studies exploring the potential role of ROS as signal molecules. Due to short life‐times and diffusion distances of ROS, these tasks require highly reactive and selective indicator reagents, localized at the presumed site of production. In the present study, we compared four double sensors: ROS indicator reagents in which partial fluorescence quenching of a dansyl moiety occurs as a result of nitroxide radical formation from a sterically hindered amine constituent. Our experiments support the idea that shorter donor‐acceptor distances within these molecules result in higher reactivity to ROS. The presence of a diethylaminoethyl side chain resulted in better selectivity to singlet oxygen: reagents lacking such substituent had an additional reactivity to superoxide anions, probably as a result of the formation of zwitterionic structures. Fluorescence localization studies of the indicator reagents in tobacco leaves and in Chlamydomonas cells show promising perspectives of their applications to plant stress studies.

Url:

https://api.istex.fr/document/AB8F0F1D4BB99A5DD2A4F3E1146452FB1D8992EA/fulltext/pdf

DOI: 10.1562/2006-02-06-RA-797

Affiliations:

Hongrie

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acceptor side</term>
<term>Additional reactivity</term>
<term>Arabidopsis thaliana</term>
<term>Better accessibility</term>
<term>Cell compartments</term>
<term>Cellular damage</term>
<term>Centre</term>
<term>Charge recombination</term>
<term>Chemical synthesis</term>
<term>Chlamydomonas</term>
<term>Chlamydomonas cells</term>
<term>Chlamydomonas reinhardii</term>
<term>Chlamydornonas cells</term>
<term>Chlorophyll</term>
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<term>Diagonal lines</term>
<term>Diethylaminoethyl side chain</term>
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<term>Mesophyll cells</term>
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<term>Organic phase</term>
<term>Other factors</term>
<term>Other hand</term>
<term>Oxalate salt</term>
<term>Oxidized form</term>
<term>Partial fluorescence quenching</term>
<term>Photobiol</term>
<term>Photochem</term>
<term>Photodynamic reaction</term>
<term>Photoinhibition</term>
<term>Photoinhibitory conditions</term>
<term>Photosensitized reaction</term>
<term>Photosynthesis</term>
<term>Photosynthetic</term>
<term>Photosystem</term>
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<term>Plant physiol</term>
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<term>Present study</term>
<term>Present work</term>
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<term>Pyrrolidine ring</term>
<term>Pyrroline ring</term>
<term>Quenching</term>
<term>Reaction centre chlorophyll</term>
<term>Reactive</term>
<term>Reactive oxygen species</term>
<term>Reagent</term>
<term>Sensor</term>
<term>Side chain</term>
<term>Singlet</term>
<term>Singlet oxygen</term>
<term>Singlet oxygen detection</term>
<term>Singlet oxygen production</term>
<term>Stress conditions</term>
<term>Structural difference</term>
<term>Superoxide anion radicals</term>
<term>Superoxide anions</term>
<term>Thylakoid</term>
<term>Thylakoid membrane</term>
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<term>Title compound</term>
<term>Triplet</term>
<term>Vas</term>
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<term>Better accessibility</term>
<term>Cell compartments</term>
<term>Cellular damage</term>
<term>Centre</term>
<term>Charge recombination</term>
<term>Chemical synthesis</term>
<term>Chlamydomonas</term>
<term>Chlamydomonas cells</term>
<term>Chlamydomonas reinhardii</term>
<term>Chlamydornonas cells</term>
<term>Chlorophyll</term>
<term>Chlorophyll fluorescence</term>
<term>Danepy</term>
<term>Diagonal lines</term>
<term>Diethylaminoethyl side chain</term>
<term>Equiv</term>
<term>Excitation</term>
<term>Flash column chromatography</term>
<term>Fluorescence</term>
<term>Fluorescence emission spectra</term>
<term>Fluorescence quenching</term>
<term>Hideg</term>
<term>Hydroxyl radicals</term>
<term>Image size</term>
<term>Indicator</term>
<term>Indicator fluorescence</term>
<term>Indicator reagents</term>
<term>Karl zeiss</term>
<term>Leaf tissue</term>
<term>Merck kieselgel</term>
<term>Mesophyll cells</term>
<term>Mmol</term>
<term>Organic phase</term>
<term>Other factors</term>
<term>Other hand</term>
<term>Oxalate salt</term>
<term>Oxidized form</term>
<term>Partial fluorescence quenching</term>
<term>Photobiol</term>
<term>Photochem</term>
<term>Photodynamic reaction</term>
<term>Photoinhibition</term>
<term>Photoinhibitory conditions</term>
<term>Photosensitized reaction</term>
<term>Photosynthesis</term>
<term>Photosynthetic</term>
<term>Photosystem</term>
<term>Plant cell physiol</term>
<term>Plant physiol</term>
<term>Pmol</term>
<term>Present study</term>
<term>Present work</term>
<term>Protein degradation</term>
<term>Pyrrolidine ring</term>
<term>Pyrroline ring</term>
<term>Quenching</term>
<term>Reaction centre chlorophyll</term>
<term>Reactive</term>
<term>Reactive oxygen species</term>
<term>Reagent</term>
<term>Sensor</term>
<term>Side chain</term>
<term>Singlet</term>
<term>Singlet oxygen</term>
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<term>Stress conditions</term>
<term>Structural difference</term>
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<front><div type="abstract" xml:lang="en">Direct detection of reactive oxygen species (ROS), especially singlet oxygen, in plants under stress conditions is of special importance, not only to identify primary events of oxidative damage, but also in studies exploring the potential role of ROS as signal molecules. Due to short life‐times and diffusion distances of ROS, these tasks require highly reactive and selective indicator reagents, localized at the presumed site of production. In the present study, we compared four double sensors: ROS indicator reagents in which partial fluorescence quenching of a dansyl moiety occurs as a result of nitroxide radical formation from a sterically hindered amine constituent. Our experiments support the idea that shorter donor‐acceptor distances within these molecules result in higher reactivity to ROS. The presence of a diethylaminoethyl side chain resulted in better selectivity to singlet oxygen: reagents lacking such substituent had an additional reactivity to superoxide anions, probably as a result of the formation of zwitterionic structures. Fluorescence localization studies of the indicator reagents in tobacco leaves and in Chlamydomonas cells show promising perspectives of their applications to plant stress studies.</div>
</front>
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Singlet Oxygen in Plants—Its Significance and Possible Detection with Double (Fluorescent and Spin) Indicator Reagents

Singlet Oxygen in Plants—Its Significance and Possible Detection with Double (Fluorescent and Spin) Indicator Reagents

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English descriptors

Abstract

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